TNFRSF12A antibody

Catalog No. ABIN2749054

Cited in 3+ publications. The Mouse Monoclonal anti-TNFRSF12A antibody (Clone ITEM-4) (ABIN2749054) specifically detects TNFRSF12A in WB, FACS and IHC (fro). The antibody is reactive with Human and Mouse samples.

Quick Overview for TNFRSF12A antibody (ABIN2749054)

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF12A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ITEM-4

Product Details anti-TNFRSF12A Antibody

Purpose: Anti-Hu CD266 Purified

Cross-Reactivity (Details): Human, Mouse

Characteristics: The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: human CD266-transfected P815 cells

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNFRSF12A antibody (ABIN2749054)

Yoriki, Akashi, Sho, Nomi, Yamato, Hotta, Takayama, Matsumoto, Wakatsuki, Migita, Yagita, Nakajima: "Therapeutic potential of the TWEAK/Fn14 pathway in intractable gastrointestinal cancer." in: Experimental and therapeutic medicine, Vol. 2, Issue 1, pp. 103-108, (2012) (PubMed).

Roos, Wicovsky, Müller, Salzmann, Rosenthal, Kalthoff, Trauzold, Seher, Henkler, Kneitz, Wajant: "Soluble and transmembrane TNF-like weak inducer of apoptosis differentially activate the classical and noncanonical NF-kappa B pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 3, pp. 1593-605, (2010) (PubMed).

Nakayama, Ishidoh, Kojima, Harada, Kominami, Okumura, Yagita: "Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 341-8, (2002) (PubMed).

Target Details for TNFRSF12A

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Alternative Name: CD266

Background: TNF receptor superfamily member 12A,CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.,TWEAKR, FN14, TNFRSF12A

Gene ID: 51330

UniProt: Q9NP84

Pathways: Apoptosis, Regulation of Cell Size