KRT8 antibody (FITC)

Catalog No. ABIN2749081

Cited in 1 publication. The FITC-conjugated Mouse Monoclonal anti-KRT8 antibody (Clone C-43) (ABIN2749081) specifically detects KRT8 in ICFC. The antibody is reactive with Human, Cow, Rabbit, Pig and Sheep samples.

Quick Overview for KRT8 antibody (FITC) (ABIN2749081)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Cow, Rabbit, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is conjugated to FITC

Application: Intracellular Flow Cytometry (ICFC)

Clone: C-43

Product Details anti-KRT8 Antibody (FITC)

Purpose: Anti-Cytokeratin 8 FITC

Specificity: The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

No Cross-Reactivity: Chicken, Hamster, Mouse, Rat, Xenopus laevis

Cross-Reactivity (Details): Sheep, Human, Bovine, Rabbit, Porcine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-2 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-KRT8 antibody (FITC) (ABIN2749081)

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Keratin 8,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K8, CK8, CYK8, KRT8, K2C8, CARD2

Gene ID: 3856

UniProt: Q7L4M3