kappa Light Chain antibody (Biotin)

Catalog No. ABIN2749092

There are 5+ publications for this product available. The Mouse Monoclonal anti-kappa Light Chain antibody is suitable to detect kappa Light Chain in samples from Human. It has been validated for FACS.

Quick Overview for kappa Light Chain antibody (Biotin) (ABIN2749092)

Target: kappa Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This kappa Light Chain antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: TB28-2

Product Details anti-kappa Light Chain Antibody (Biotin)

Purpose: Anti-Hu Ig Kappa Light Chain Biotin

Cross-Reactivity (Details): Human

Characteristics: The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Human IgG-kappa myeloma protein

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-12 μg/mL. Extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-kappa Light Chain antibody (Biotin) (ABIN2749092)

Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

Böttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).

Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).

Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).

Kiyotaki, Cooper, Bertoli, Kearney, Kubagawa: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, (1987) (PubMed).

Target Details for kappa Light Chain

Target: kappa Light Chain

Alternative Name: Ig Kappa Light Chain

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk

Gene ID: 35