CD57 antibody

Catalog No. ABIN2763751

This Mouse Monoclonal antibody specifically detects CD57 in IHC, WB, IF and IP. It exhibits reactivity toward Human.

Quick Overview for CD57 antibody (ABIN2763751)

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: NK-1

Product Details anti-CD57 Antibody

Purpose: Mouse anti Human CD57

Cross-Reactivity: Human

Characteristics: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced from mice immunized with human peripheral blood mononuclear cells.

Isotype: IgM

Application Details

Application Notes: Antibody can be used for Western blotting (1-10 μg/mL), Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Immunofluorescence (2-20 μg/mL), Immunohistochemistry (2-20 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Alternative Name: CD57

Background: HNK1, GLCATP, NK-1, HNK-1, LEU7, NK1, 151290, B3GAT1, Q9P2W7, GlcUAT-P, CD57, GlcUATP, GlcAT-P, 27087, NK 1, HNK 1, GlcAT P, GlcUAT P

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process