Selenoprotein N antibody (C-Term)

Catalog No. ABIN2773903

The Rabbit Polyclonal anti-Selenoprotein N antibody (ABIN2773903) specifically detects Selenoprotein N in WB and IHC. The antibody is reactive with Human, Mouse, Zebrafish (Danio rerio), Rat, Horse, Guinea Pig and Cow samples.

Quick Overview for Selenoprotein N antibody (C-Term) (ABIN2773903)

Target: Selenoprotein N (SELENON)

Reactivity: Human, Mouse, Zebrafish (Danio rerio), Rat, Horse, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Selenoprotein N antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Selenoprotein N Antibody

Binding Specificity: C-Term

Purpose: SELENON Antibody - C-terminal region

Sequence: ANYFLDITSV KPEEIESNLF SFSSTFEDPS TATYMQFLKE GLRRGLPLLQ

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against SEPN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SEPN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47655-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Selenoprotein N

Target: Selenoprotein N (SELENON)

Alternative Name: SELENON

Background:

Background Information: This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in this gene are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. SEPN1-related myopathy consists of 4 autosomal recessive disorders, originally thought to be separate entities: rigid spine muscular dystrophy (RSMD1), the classical form of multiminicore disease, desmin related myopathy with Mallory-body like inclusions, and congenital fiber-type disproportion (CFTD). This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. Alternatively spliced transcript variants have been found for this gene.

Gene Name: selenoprotein N

Alternative Symbols: RSS, CFTD, SELN, MDRS1, RSMD1, SEPN1

Protein Name: selenoprotein N

Molecular Weight: 66 kDa

Gene ID: 57190

NCBI Accession: NP_065184

UniProt: Q9NZV5