Adenylate Kinase 1 antibody (Middle Region)

Catalog No. ABIN2773912

The Rabbit Polyclonal anti-Adenylate Kinase 1 antibody is suitable to detect Adenylate Kinase 1 in samples from Human, Mouse, Rat, Rabbit, Dog, Cow, Sheep, Guinea Pig, Horse, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for Adenylate Kinase 1 antibody (Middle Region) (ABIN2773912)

Target: Adenylate Kinase 1 (AK1)

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Sheep, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenylate Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Adenylate Kinase 1 Antibody

Binding Specificity: Middle Region

Purpose: AK1 Antibody - middle region

Sequence: RIGQPTLLLY VDAGPETMTQ RLLKRGETSG RVDDNEETIK KRLETYYKAT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against AK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AK1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48149-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Adenylate Kinase 1

Target: Adenylate Kinase 1 (AK1)

Alternative Name: AK1

Background:

Background Information: Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Adenylate kinase 1

Alternative Symbols: HTL-S-58j

Protein Name: Adenylate kinase isoenzyme 1

Molecular Weight: 22kDa

Gene ID: 203

NCBI Accession: NP_000467

UniProt: P00568

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process