FZR1 antibody (N-Term)

Catalog No. ABIN2773984

The Rabbit Polyclonal anti-FZR1 antibody has been validated for WB. It is suitable to detect FZR1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio) and Pig.

Quick Overview for FZR1 antibody (N-Term) (ABIN2773984)

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FZR1 Antibody

Binding Specificity: N-Term

Sequence: RQIIIQNENT VPCVSEMRRT LTPANSPVSS PSKHGDRFIP SRAGANWSVN

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rat: 93%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against Fzr1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 493 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: Fzr1

Background: Fzr1 is the key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Fzr1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Alias Symbols: AW108046, Cdh1, FZR, FZR2, Fyr, HCDH, HCDH1
Protein Interaction Partner: Arhgap35, Rrm2, Ccnb1, Cdc27, Sirt2, Smurf1, Skil, Ube2c, Csf3r,
Protein Size: 493

Molecular Weight: 55 kDa

Gene ID: 56371

NCBI Accession: NM_019757, NP_062731

UniProt: Q9R1K5

Pathways: DNA Replication, Synthesis of DNA