PNKP antibody (N-Term)

Catalog No. ABIN2774011

The Rabbit Polyclonal anti-PNKP antibody (ABIN2774011) specifically detects PNKP in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig and Horse samples.

Quick Overview for PNKP antibody (N-Term) (ABIN2774011)

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PNKP Antibody

Binding Specificity: N-Term

Purpose: PNKP Antibody - N-terminal region

Sequence: MGEVEAPGRL WLESPPGGAP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRTQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PNKP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PNKP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52311-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP

Background:

Background Information: This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

Gene Name: Polynucleotide kinase 3'-phosphatase

Alternative Symbols: PNK, AOA4, MCSZ, CMT2B2, EIEE10

Protein Name: Bifunctional polynucleotide phosphatase/kinase

Molecular Weight: 57kDa

Gene ID: 11284

NCBI Accession: NP_009185

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation