ATG12 antibody (Middle Region)

Catalog No. ABIN2774065

The Rabbit Polyclonal anti-ATG12 antibody is suitable to detect ATG12 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for ATG12 antibody (Middle Region) (ABIN2774065)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG12 Antibody

Binding Specificity: Middle Region

Purpose: ATG12 Antibody - middle region

Sequence: KFLKLVASEQ LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ATG12. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54265-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background:

Background Information: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).

Gene Name: ATG12 autophagy related 12 homolog (S. cerevisiae)

Alternative Symbols: APG12, FBR93, APG12L, HAPG12

Protein Name: Ubiquitin-like protein ATG12

Molecular Weight: 21kDa

Gene ID: 9140

NCBI Accession: NP_004698

UniProt: O94817

Pathways: Autophagy