CHMP2B antibody (N-Term)

Catalog No. ABIN2774093

This Rabbit Polyclonal antibody specifically detects CHMP2B in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for CHMP2B antibody (N-Term) (ABIN2774093)

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHMP2B Antibody

Binding Specificity: N-Term

Sequence: ASLFKKKTVD DVIKEQNREL RGTQRAIIRD RAALEKQEKQ LELEIKKMAK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against Chmp2b. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Rat Chmp2b

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 213 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHMP2B

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Alternative Name: Chmp2b

Background: Chmp2b is involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Chmp2b recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Chmp2b redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery Chmp2b also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis .
Alias Symbols: RGD1306781
Protein Size: 213

Molecular Weight: 23 kDa

Gene ID: 363720

NCBI Accession: XM_001063932, XP_001063932