Mre11 antibody (N-Term)

Catalog No. ABIN2774155

The Rabbit Polyclonal anti-Mre11 antibody is suitable to detect Mre11 in samples from Human, Rat, Mouse, Dog, Rabbit, Saccharomyces cerevisiae, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio) and Goat. It has been validated for WB.

Quick Overview for Mre11 antibody (N-Term) (ABIN2774155)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Saccharomyces cerevisiae, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mre11 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Mre11 Antibody

Binding Specificity: N-Term

Purpose: MRE11 Antibody - N-terminal region

Sequence: DTFVTLDEIL RLAQENEVDF ILLGGDLFHE NKPSRKTLHT CLELLRKYCM

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against MRE11A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRE11A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56644-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Alternative Name: MRE11

Background:

Background Information: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Gene Name: MRE11 homolog, double strand break repair nuclease

Alternative Symbols: ATLD, HNGS1, MRE11A, MRE11B

Protein Name: double-strand break repair protein MRE11

Molecular Weight: 80kDa

Gene ID: 4361

NCBI Accession: NP_005582

UniProt: P49959

Pathways: DNA Damage Repair