RNF139 antibody (N-Term)

Catalog No. ABIN2774685

This Rabbit Polyclonal antibody specifically detects RNF139 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Horse.

Quick Overview for RNF139 antibody (N-Term) (ABIN2774685)

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF139 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF139 Antibody

Binding Specificity: N-Term

Purpose: RNF139 Antibody - N-terminal region

Sequence: SQRSLFKFYT YSSAFLLAAT SVLVNYYASL HIDFYGAYNT SAFGIELLPR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 87%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF139. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RNF139

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43092-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF139

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Alternative Name: RNF139

Background:

Background Information: RNF139 is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Gene Name: Ring finger protein 139

Alternative Symbols: RCA1, TRC8, HRCA1

Protein Name: E3 ubiquitin-protein ligase RNF139

Molecular Weight: 76 kDa

Gene ID: 11236

NCBI Accession: NP_009149

UniProt: Q8WU17