MKRN1 antibody (N-Term)

Catalog No. ABIN2774707

This Rabbit Polyclonal antibody specifically detects MKRN1 in WB. It exhibits reactivity toward Human, Mouse, Dog, Guinea Pig, Horse, Cow, Rat, Rabbit and Zebrafish (Danio rerio).

Quick Overview for MKRN1 antibody (N-Term) (ABIN2774707)

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Cow, Rat, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKRN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MKRN1 Antibody

Binding Specificity: N-Term

Purpose: MKRN1 Antibody - N-terminal region

Sequence: GDRCRYEHSK PLKQEEATAT ELTTKSSLAA SSSLSSIVGP LVEMNTGEAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 83%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against MKRN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MKRN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104798

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MKRN1

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Alternative Name: MKRN1

Background:

Background Information: The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Gene Name: Makorin ring finger protein 1

Alternative Symbols: RNF61

Protein Name: E3 ubiquitin-protein ligase makorin-1

Molecular Weight: 16kDa

Gene ID: 23608

NCBI Accession: NP_038474

UniProt: Q9UHC7

Pathways: Chromatin Binding