MARCH5 antibody (C-Term)

Catalog No. ABIN2774747

The Rabbit Polyclonal anti-MARCH5 antibody (ABIN2774747) specifically detects MARCH5 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse and Rabbit samples.

Quick Overview for MARCH5 antibody (C-Term) (ABIN2774747)

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH5 Antibody

Binding Specificity: C-Term

Sequence: VNSNLQRTIL GGIAFVAIKG AFKVYFKQQQ YLRQAHRKIL NYPEQEEA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against 39877. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MARCH5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 278 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH5

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Alternative Name: MARCH5

Background: MARCH5 is an ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2) and DRP1 (DNM1L).MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2, MIM 608507) and DRP1 (DNM1L, MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1502 AK000452.1 3-1504 1503-1863 BC015480.1 1463-1823 1864-1901 BE393612.1 438-475 1902-3713 BC015480.1 1852-3663 3714-3941 BC015480.1 3669-3896
Alias Symbols: FLJ20445, MARCH-V, MITOL, RNF153
Protein Interaction Partner: FATE1, UBE2W, UBC, DNM1L, MFN2, UBE2E1, FIS1, UBE2E3, MFN1, PARK2, MARCH5, CCNB1, UBE2D3, UBE2D2, UBE2D1, MAP1B, KIAA0368, SOD1, MAVS, TANK, TRAF6, AKTIP, UBE2T, UBE2N, UBE2I, UTRN,
Protein Size: 278

Molecular Weight: 31 kDa

Gene ID: 54708

NCBI Accession: NM_017824, NP_060294

UniProt: Q9NX47