MARCH5 antibody (C-Term)

Catalog No. ABIN6738065

Product Details anti-MARCH5 Antibody

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken, Dog, Horse, Rabbit, Guinea Pig, Monkey, Bat, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human MARCH5

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MARCH5 (Q9NX47, NP_060294). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish (100%), Trout, Salmon, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MARCH5

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Alternative Name: MARCH5 (MARCH5 Products)

Synonyms: march5 antibody, MARCH5 antibody, mitol antibody, rnf153 antibody, march-v antibody, zgc:162966 antibody, MARCH-V antibody, Mar-05 antibody, MITOL antibody, RNF153 antibody, 1810015H18Rik antibody, 2310008I22Rik antibody, 2700055A20Rik antibody, 5730499H23Rik antibody, E130202O05Rik antibody, Marchv antibody, Rnf153 antibody, membrane-associated ring finger (C3HC4) 5 antibody, membrane associated ring-CH-type finger 5 antibody, membrane associated ring-CH-type finger 5 L homeolog antibody, march5 antibody, MARCH5 antibody, march5.L antibody, March5 antibody

Background: Name/Gene ID: MARCH5

Synonyms: MARCH5, MARCH-V, RING finger protein 153, RNF153, Mitochondrial ubiquitin ligase, MITOL

Gene ID: 54708

NCBI Accession: NP_060294

UniProt: Q9NX47