CHFR antibody (N-Term)

Catalog No. ABIN2774757

The Rabbit Polyclonal anti-CHFR antibody has been validated for WB and IHC. It is suitable to detect CHFR in samples from Human, Mouse, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog and Horse.

Quick Overview for CHFR antibody (N-Term) (ABIN2774757)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHFR Antibody

Binding Specificity: N-Term

Sequence: REWTIGRRRG CDLSFPSNKL VSGDHCRIVV DEKSGQVTLE DTSTSGTVIN

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CHFR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 623 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR

Background: CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Alias Symbols: FLJ10796, FLJ33629, RNF116, RNF196
Protein Interaction Partner: USP7, VIM, UBE2D2, AURKA, HLTF, RANBP2, PLK1, PCNA, MCM2, HDAC2, HDAC1, CDK5, CDC20, PARP1, SIN3A, CHFR, UBC13, SUMO1, UBE2I, LRP1, UBE2V1, UBE2N, UBE2D1, TRIM9, RNF125, CBLC, RNF7, RELA, RNF8, HIST2H2BE, HIST2H2AC, UBC, SMARCD1, SMARCB1, SMARCA4, KIF22,
Protein Size: 623

Molecular Weight: 69 kDa

Gene ID: 55743

NCBI Accession: NM_018223, NP_060693

UniProt: Q96EP1