GJB2 antibody (N-Term)

Catalog No. ABIN2774858

The Rabbit Polyclonal anti-GJB2 antibody is suitable to detect GJB2 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Sheep. It has been validated for WB.

Quick Overview for GJB2 antibody (N-Term) (ABIN2774858)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GJB2 Antibody

Binding Specificity: N-Term

Purpose: GJB2 Antibody - middle region

Sequence: STPALLVAMH VAYRRHEKKR KFIKGEIKSE FKDIEEIKTQ KVRIEGSLWW

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 79%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against GJB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GJB2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36608-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2

Background:

Background Information: Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1, MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.

Gene Name: Gap junction protein, beta 2, 26 kDa

Alternative Symbols: HID, KID, PPK, BAPS, CX26, DFNA3, DFNB1, NSRD1, DFNA3A, DFNB1A

Protein Name: Gap junction beta-2 protein

Molecular Weight: 25kDa

Gene ID: 2706

NCBI Accession: NP_003995

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization