MCM7 antibody (Middle Region)

Catalog No. ABIN2774887

The Rabbit Polyclonal anti-MCM7 antibody has been validated for WB and IHC. It is suitable to detect MCM7 in samples from Human, Rat, Rabbit, Guinea Pig, Cow and Horse.

Quick Overview for MCM7 antibody (Middle Region) (ABIN2774887)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human, Rat, Rabbit, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MCM7 Antibody

Binding Specificity: Middle Region

Sequence: HRIVKMNKSE DDESGAGELT REELRQIAEE DFYEKLAASI APEIYGHEDV

Predicted Reactivity: Cow: 92%, Guinea Pig: 85%, Horse: 92%, Human: 100%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against MCM7. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MCM7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 719 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background: MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3
Protein Interaction Partner: AK8, MIPOL1, C8orf34, USHBP1, MCMBP, CCDC102B, TRIM54, MBIP, UBQLN1, TRIM27, NAB2, KIFC3, GOLGA2, PNMA1, UBC, SP2, HAUS2, CEP250, CEP57, SUMO2, SUMO3, PCNA, STAU1, SUZ12, RNF2, MCM4, MCM2, HSP90AB1, PRMT1, PRRC1, CLUH, TOMM34, TRIM16, HYOU1, BAG3, MIB1, P
Protein Size: 719

Molecular Weight: 81 kDa

Gene ID: 4176

NCBI Accession: NM_005916, NP_005907

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA