MCM7 antibody (N-Term)

Catalog No. ABIN2774888

The Rabbit Polyclonal anti-MCM7 antibody (ABIN2774888) specifically detects MCM7 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Cow and Horse samples.

Quick Overview for MCM7 antibody (N-Term) (ABIN2774888)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM7 Antibody

Binding Specificity: N-Term

Purpose: MCM7 Antibody - N-terminal region

Sequence: MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MCM7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36653-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background:

Background Information: MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB.

Gene Name: Minichromosome maintenance complex component 7

Alternative Symbols: MCM2, CDC47, P85MCM, P1CDC47, PNAS146, PPP1R104, P1.1-MCM3

Protein Name: DNA replication licensing factor MCM7

Molecular Weight: 42kDa

Gene ID: 4176

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA