Osteopontin antibody (C-Term)

Catalog No. ABIN2774904

This anti-Osteopontin antibody is a Rabbit Polyclonal antibody detecting Osteopontin in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Goat and Sheep. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Osteopontin antibody (C-Term) (ABIN2774904)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Osteopontin Antibody

Binding Specificity: C-Term

Sequence: DSQELSKVSR EFHSHEFHSH EDMLVVDPKS KEEDKHLKFR ISHELDSASS

Predicted Reactivity: Cow: 86%, Dog: 79%, Goat: 79%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Rat: 79%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against SPP1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SPP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 300 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Osteopontin antibody (ABIN2774904)

Spataru, Cojocaru, Sandu, Solcan, Duceac, Baltatu, Voiculescu, Geanta, Vizureanu: "Assessment of the Effects of Si Addition to a New TiMoZrTa System." in: Materials (Basel, Switzerland), Vol. 14, Issue 24, (2021) (PubMed).

Baltatu, Spataru, Verestiuc, Balan, Solcan, Sandu, Geanta, Voiculescu, Vizureanu: "Design, Synthesis, and Preliminary Evaluation for Ti-Mo-Zr-Ta-Si Alloys for Potential Implant Applications." in: Materials (Basel, Switzerland), Vol. 14, Issue 22, (2021) (PubMed).

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background: The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.
Alias Symbols: OPN, BNSP, BSPI, ETA-1
Protein Interaction Partner: SGTA, MEOX2, PARP1, BAG6, PEPD, APOA1, UBQLN4, CISD2, TUBB, CC2D1B, COMMD1, RIMS4, C2orf50, CMTM3, DSEL, ZNF804A, USP42, TMEM168, LRRC4, GRIPAP1, TMEM30A, AMOTL2, C1RL, DNTTIP2, KCNIP1, STK39, EIF3K, ZNF638, AFF4, CACNG4, SLC39A6, PLD3, SNAPIN, NCSTN, CEP
Protein Size: 300

Molecular Weight: 33 kDa

Gene ID: 6696

NCBI Accession: NM_000582, NP_000573

Pathways: Regulation of Cell Size