IBSP antibody (N-Term)

Catalog No. ABIN2774907

The Rabbit Polyclonal anti-IBSP antibody (ABIN2774907) specifically detects IBSP in WB. The antibody is reactive with Human and Dog samples.

Quick Overview for IBSP antibody (N-Term) (ABIN2774907)

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IBSP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IBSP Antibody

Binding Specificity: N-Term

Purpose: IBSP Antibody - N-terminal region

Sequence: SATTLGYGED ATPGTGYTGL AAIQLPKKAG DITNKATKEK ESDEEEEEEE

Predicted Reactivity: Dog: 79%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against IBSP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IBSP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103195

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Alternative Name: IBSP

Background:

Background Information: The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Gene Name: Integrin-binding sialoprotein

Alternative Symbols: BSP, BNSP, SP-II, BSP-II

Protein Name: Bone sialoprotein 2

Molecular Weight: 35kDa

Gene ID: 3381

NCBI Accession: NP_004958

UniProt: P21815