NEDD4 antibody (Middle Region)

Catalog No. ABIN2774920

The Rabbit Polyclonal anti-NEDD4 antibody (ABIN2774920) specifically detects NEDD4 in WB. The antibody is reactive with Human, Rat, Mouse, Horse, Dog, Rabbit, Cow and Guinea Pig samples.

Quick Overview for NEDD4 antibody (Middle Region) (ABIN2774920)

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Reactivity: Human, Rat, Mouse, Horse, Dog, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEDD4 Antibody

Binding Specificity: Middle Region

Sequence: SRRGSLQAYT FEEQPTLPVL LPTSSGLPPG WEEKQDERGR SYYVDHNSRT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NEDD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NEDD4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 900 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Alternative Name: NEDD4

Background: NEDD4 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4 is involved in the budding of many viruses.
Alias Symbols: KIAA0093, MGC176705, RPF1, NEDD4-1
Protein Interaction Partner: RPO21, UBI4, UBC8, RNF7, SRC, POLR2A, NEDD4, CUL5, UBE2L3, UBE2D3, UBE2D1, UBC, FGFR1, SPT23, PTCH1, SMO, YOD1, TNK2, SNCA, GUCD1, FBXL18, ERBB3, CSNK1D, CCNH, RBX1, BTRC, CUL1, SKP1, PTEN, ARRDC3, MDM2, RAPGEF2, AMOTL1, POLR2M, RPAP2, EPRS, DIAPH1, DDX3X
Protein Size: 900

Molecular Weight: 104 kDa

Gene ID: 4734

NCBI Accession: NM_006154, NP_006145

UniProt: B4E2R7

Pathways: Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2