AKAP5 antibody (Middle Region)

Catalog No. ABIN2775174

The Rabbit Polyclonal anti-AKAP5 antibody (ABIN2775174) specifically detects AKAP5 in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Horse, Pig and Saccharomyces cerevisiae samples.

Quick Overview for AKAP5 antibody (Middle Region) (ABIN2775174)

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKAP5 Antibody

Binding Specificity: Middle Region

Purpose: AKAP5 Antibody - middle region

Sequence: KQFLISAENE QVGVFANDNG FEDRTSEQYE TLLIETASSL VKNAIQLSIE

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 79%, Yeast: 82%

Characteristics: This is a rabbit polyclonal antibody against AKAP5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKAP5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36322-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for AKAP5

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Alternative Name: AKAP5

Background:

Background Information: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP5 is a member of the AKAP family. It binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: A kinase (PRKA) anchor protein 5

Alternative Symbols: H21, AKAP75, AKAP79

Protein Name: A-kinase anchor protein 5

Molecular Weight: 47kDa

Gene ID: 9495

NCBI Accession: NP_004848

UniProt: P24588

Pathways: cAMP Metabolic Process