RENT1/UPF1 antibody (Middle Region)

Catalog No. ABIN2775184

The Rabbit Polyclonal anti-RENT1/UPF1 antibody is suitable to detect RENT1/UPF1 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig and Horse. It has been validated for WB and IHC.

Quick Overview for RENT1/UPF1 antibody (Middle Region) (ABIN2775184)

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RENT1/UPF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RENT1/UPF1 Antibody

Binding Specificity: Middle Region

Purpose: UPF1 Antibody - middle region

Sequence: RGTPKGKTGR GGRQKNRFGL PGPSQTNLPN SQASQDVASQ PFSQGALTQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 93%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against UPF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UPF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36350-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Alternative Name: UPF1

Background:

Background Information: UPF1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: UPF1 regulator of nonsense transcripts homolog (yeast)

Alternative Symbols: UTF, HUPF1, NORF1, RENT1, smg-2, pNORF1

Protein Name: Regulator of nonsense transcripts 1

Molecular Weight: 123kDa

Gene ID: 5976

NCBI Accession: NP_002902

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome