RAD54L antibody (N-Term)

Catalog No. ABIN2775186

This Rabbit Polyclonal antibody specifically detects RAD54L in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat and Zebrafish (Danio rerio).

Quick Overview for RAD54L antibody (N-Term) (ABIN2775186)

Target: RAD54L (RAD54-Like (RAD54L))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54L antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD54L Antibody

Binding Specificity: N-Term

Purpose: RAD54L Antibody - N-terminal region

Sequence: VVSPSSLVKN WYNEVGKWLG GRIQPLAIDG GSKDEIDQKL EGFMNQRGAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RAD54L. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RAD54L

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36355-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD54L

Target: RAD54L (RAD54-Like (RAD54L))

Alternative Name: RAD54L

Background:

Background Information: This protein belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination.The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: RAD54-like (S. cerevisiae)

Alternative Symbols: HR54, hHR54, RAD54A, hRAD54

Protein Name: DNA repair and recombination protein RAD54-like

Molecular Weight: 84kDa

Gene ID: 8438

NCBI Accession: NP_003570

UniProt: Q92698

Pathways: DNA Damage Repair