Perforin 1 antibody (N-Term)

Catalog No. ABIN2775294

Product Details anti-Perforin 1 Antibody

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Pig, Horse, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SVAGSHSQAA NFAAQKTHQD QYSFSTDTVE CRFYSFHVVH TPPLHPDFKR

Predicted Reactivity: Cow: 77%, Dog: 92%, Guinea Pig: 77%, Horse: 85%, Human: 100%, Pig: 100%, Rabbit: 92%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against PRF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 555 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1 (PRF1 Products)

Synonyms: FLH2 antibody, HPLH2 antibody, P1 antibody, PFN1 antibody, PFP antibody, PRF1 antibody, Pfn antibody, Pfp antibody, Prf-1 antibody, Cyta antibody, RATCYTA antibody, LOC443187 antibody, perforin antibody, prf1 antibody, cytolysin antibody, perforin-1 antibody, perforin-1-like antibody, perforin 1 antibody, perforin 1 (pore forming protein) antibody, perforin antibody, perforin 1 L homeolog antibody, PRF1 antibody, Prf1 antibody, LOC443187 antibody, prf1 antibody, prf1.L antibody

Background: PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the gene encoding PRF1 cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
Alias Symbols: FLH2, HPLH2, MGC65093, P1, PFP, PFN1
Protein Interaction Partner: KRT31, FBXO6, PRF1, TRIM54, DDX24, GZMB, CALR,
Protein Size: 555

Molecular Weight: 59 kDa

Gene ID: 5551

NCBI Accession: NM_005041, NP_005032

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis