RAD23A antibody (N-Term)

Catalog No. ABIN2775295

The Rabbit Polyclonal anti-RAD23A antibody is suitable to detect RAD23A in samples from Human, Mouse, Rat, Dog, Horse, Rabbit, Cow and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for RAD23A antibody (N-Term) (ABIN2775295)

Target: RAD23A (RAD23 Homolog A (RAD23A))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD23A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAD23A Antibody

Binding Specificity: N-Term

Purpose: RAD23A Antibody - N-terminal region

Sequence: VPSSGSSGRE EDAASTLVTG SEYETMLTEI MSMGYERERV VAALRASYNN

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RAD23A. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RAD23A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109909

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD23A

Target: RAD23A (RAD23 Homolog A (RAD23A))

Alternative Name: RAD23A

Background:

Background Information: RAD23A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Gene Name: RAD23 homolog A (S. cerevisiae)

Alternative Symbols: HR23A, HHR23A

Protein Name: UV excision repair protein RAD23 homolog A

Molecular Weight: 40kDa

Gene ID: 5886

NCBI Accession: NP_005044

UniProt: P54725

Pathways: DNA Damage Repair