Mesothelin antibody (Middle Region)

Catalog No. ABIN2775327

The Rabbit Polyclonal anti-Mesothelin antibody is suitable to detect Mesothelin in samples from Human, Mouse, Rat, Cow, Guinea Pig, Pig, Dog and Horse. It has been validated for WB and IHC.

Quick Overview for Mesothelin antibody (Middle Region) (ABIN2775327)

Target: Mesothelin (MSLN)

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Pig, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Mesothelin Antibody

Binding Specificity: Middle Region

Purpose: MSLN Antibody - middle region

Sequence: QKLLGPHVEG LKAEERHRPV RDWILRQRQD DLDTLGLGLQ GGIPNGYLVL

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MSLN. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MSLN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42262-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background:

Background Information: An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin.An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.

Gene Name: Mesothelin

Alternative Symbols: MPF, SMRP

Protein Name: Mesothelin

Molecular Weight: 69 kDa

Gene ID: 10232

NCBI Accession: NP_005814

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process