GAPVD1 antibody (N-Term)

Catalog No. ABIN2775423

The Rabbit Polyclonal anti-GAPVD1 antibody is suitable to detect GAPVD1 in samples from Human, Mouse, Dog, Rat, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for GAPVD1 antibody (N-Term) (ABIN2775423)

Target: GAPVD1 (GTPase Activating Protein and VPS9 Domains 1 (GAPVD1))

Reactivity: Human, Mouse, Dog, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPVD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GAPVD1 Antibody

Binding Specificity: N-Term

Purpose: GAPVD1 Antibody - N-terminal region

Sequence: FKLFSEGLFS AKLFLTATLH EPIMQLLVED EDHLETDPNK LIERFSPSQQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against GAPVD1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPVD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102011

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GAPVD1

Target: GAPVD1 (GTPase Activating Protein and VPS9 Domains 1 (GAPVD1))

Alternative Name: GAPVD1

Background:

Background Information: GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. It has GEF activity for Rab5 and GAP activity for Ras.

Gene Name: GTPase activating protein and VPS9 domains 1

Alternative Symbols: RAP6, GAPEX5, GAPex-5

Protein Name: GTPase-activating protein and VPS9 domain-containing protein 1

Molecular Weight: 166kDa

Gene ID: 26130

NCBI Accession: NP_056450

UniProt: Q14C86