Caldesmon antibody (Middle Region)

Catalog No. ABIN502291

This Rabbit Polyclonal antibody specifically detects Caldesmon in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Horse, Rabbit and Guinea Pig.

Quick Overview for Caldesmon antibody (Middle Region) (ABIN502291)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caldesmon Antibody

Binding Specificity: Middle Region

Sequence: FSSPTAAGTP NKETAGLKVG VSSRINEWLT KTPDGNKSPA PKPSDLRPGD

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against CALD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CALD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 538 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: CALD1

Background: CALD1 is a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Alias Symbols: CDM, H-CAD, L-CAD, MGC21352, NAG22, HCAD, LCAD
Protein Interaction Partner: TP53, RPA3, RPA2, RPA1, PHAX, FH, EPB41L2, CSNK2A1, LRCH3, PRKACA, PAN2, UBC, HSP90AA1, CALM1, ACTN1, TINF2, POT1, ISG15, TERF1, ARRB1, Poc1b, Cep76, Sass6, Pafah1b1, TERF2, SHC1, CDK1, CALM3, GRB2,
Protein Size: 538

Molecular Weight: 63 kDa

Gene ID: 800

NCBI Accession: NM_004342, NP_004333

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction