ATG4A antibody (Middle Region)

Catalog No. ABIN2775554

This Rabbit Polyclonal antibody specifically detects ATG4A in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for ATG4A antibody (Middle Region) (ABIN2775554)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG4A Antibody

Binding Specificity: Middle Region

Purpose: ATG4A Antibody - middle region

Sequence: PQSLGALGGK PNNAYYFIGF LGDELIFLDP HTTQTFVDTE ENGTVNDQTF

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ATG4A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG4A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42722-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATG4A antibody (ABIN2775554)

Bortnik, Choutka, Horlings, Leung, Baker, Lebovitz, Dragowska, Go, Bally, Minchinton, Gelmon, Gorski: "Identification of breast cancer cell subtypes sensitive to ATG4B inhibition." in: Oncotarget, Vol. 7, Issue 41, pp. 66970-66988, (2016) (PubMed).

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A

Background:

Background Information: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. ATG4A is a member of the autophagin protein family. ATG4A is also designated as a member of the C-54 family of cysteine proteases.

Gene Name: ATG4 autophagy related 4 homolog A (S. cerevisiae)

Alternative Symbols: APG4A, AUTL2

Protein Name: Cysteine protease ATG4A

Molecular Weight: 45kDa

Gene ID: 115201

NCBI Accession: NP_443168

UniProt: Q8WYN0

Pathways: Autophagy