Occludin antibody (N-Term)

Catalog No. ABIN2775620

There is 1 publication for this product available. The Rabbit Polyclonal anti-Occludin antibody is suitable to detect Occludin in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Occludin antibody (N-Term) (ABIN2775620)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Occludin Antibody

Binding Specificity: N-Term

Purpose: OCLN Antibody - N-terminal region

Sequence: VRPMLSQPAY SFYPEDEILH FYKWTSPPGV IRILSMLIIV MCIAIFACVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against OCLN. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human OCLN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42889-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Occludin antibody (ABIN2775620)

Yang, Liu, Miao, Yang, Fu, Dou, Cai, Zong, Tan, Chen, Wang: "Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier." in: Oncotarget, Vol. 7, Issue 39, pp. 63839-63855, (2016) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background:

Background Information: OCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Occludin

Alternative Symbols: BLCPMG

Protein Name: Occludin

Molecular Weight: 59kDa

Gene ID: 4950

NCBI Accession: NP_002529

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C