CHD4 antibody (N-Term)

Catalog No. ABIN2775753

The Rabbit Polyclonal anti-CHD4 antibody is suitable to detect CHD4 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for CHD4 antibody (N-Term) (ABIN2775753)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHD4 Antibody

Binding Specificity: N-Term

Purpose: CHD4 Antibody - N-terminal region

Sequence: QKKERMLLCR QLGDSSGEGP EFVEEEEEVA LRSDSEGSDY TPGKKKKKKL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CHD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34372-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: CHD4

Background:

Background Information: CHD4 is a probable transcription regulator.The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Chromodomain helicase DNA binding protein 4

Alternative Symbols: CHD-4, Mi-2b, SIHIWES, Mi2-BETA

Protein Name: Chromodomain-helicase-DNA-binding protein 4

Molecular Weight: 218kDa

Gene ID: 1108

NCBI Accession: NP_001264

UniProt: Q14839