ZYX antibody (Middle Region)

Catalog No. ABIN2775767

The Rabbit Polyclonal anti-ZYX antibody has been validated for WB and IHC. It is suitable to detect ZYX in samples from Human, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for ZYX antibody (Middle Region) (ABIN2775767)

Target: ZYX (Zyxin (ZYX))

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ZYX Antibody

Binding Specificity: Middle Region

Sequence: QPRGPPASSP APAPKFSPVT PKFTPVASKF SPGAPGGSGS QPNQKLGHPE

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ZYX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZYX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 572 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: ZYX

Background: TRIM50 is an E3 ubiquitin-protein ligase.Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Alias Symbols: ESP-2, HED-2
Protein Interaction Partner: RTN4IP1, YPEL3, IMP3, PHF21A, PDLIM5, LIN7A, SUMO1, NEDD8, FAM129B, CORO1B, CAND1, PUF60, PAPOLA, NUDC, SEC23B, SEC24C, HIST1H4A, UGP2, TARS, PXN, GART, GARS, APEH, FN1, HIPK3, VCAM1, UBC, UBL7, UFC1, UBXN7, ZC3H7B, TTLL12, FERMT2, TOMM34, AHCYL1, SSSCA1,
Protein Size: 572

Molecular Weight: 61 kDa

Gene ID: 7791

NCBI Accession: NM_003461, NP_003452

UniProt: Q15942