PHD1 antibody (N-Term)

Catalog No. ABIN2775821

The Rabbit Polyclonal anti-PHD1 antibody is suitable to detect PHD1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Pig. It has been validated for WB.

Quick Overview for PHD1 antibody (N-Term) (ABIN2775821)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHD1 Antibody

Binding Specificity: N-Term

Purpose: EGLN2 Antibody - N-terminal region

Sequence: SAGSGTPRAT ATSTTASPLR DGFGGQDGGE LRPLQSEGAA ALVTKGCQRL

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 86%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EGLN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EGLN2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099493

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2

Background:

Background Information: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms.

Gene Name: Egl nine homolog 2 (C. elegans)

Alternative Symbols: EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1

Protein Name: Egl nine homolog 2

Molecular Weight: 44kDa

Gene ID: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis