HLTF antibody (N-Term)

Catalog No. ABIN2775846

The Rabbit Polyclonal anti-HLTF antibody is suitable to detect HLTF in samples from Human, Rat, Mouse, Dog, Rabbit, Cow, Horse and Pig. It has been validated for WB.

Quick Overview for HLTF antibody (N-Term) (ABIN2775846)

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLTF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLTF Antibody

Binding Specificity: N-Term

Purpose: SMARCA3 Antibody - N-terminal region

Sequence: DPVWKYLQTV QYGVHGNFPR LSYPTFFPRF EFQDVIPPDD FLTSDEEVDS

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against SMARCA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMARCA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102312

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLTF

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Alternative Name: SMARCA3

Background:

Background Information: SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform which is truncated at the N-terminus as compared to the full-length protein.

Gene Name: Helicase-like transcription factor

Alternative Symbols: ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SMARCA3

Protein Name: Helicase-like transcription factor

Molecular Weight: 114kDa

Gene ID: 6596

NCBI Accession: NP_620636

UniProt: Q14527