TRIM27 antibody (Middle Region)

Catalog No. ABIN2775961

The Rabbit Polyclonal anti-TRIM27 antibody (ABIN2775961) specifically detects TRIM27 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Guinea Pig samples.

Quick Overview for TRIM27 antibody (Middle Region) (ABIN2775961)

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM27 Antibody

Binding Specificity: Middle Region

Purpose: TRIM27 Antibody - middle region

Sequence: KRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRIM27. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM27

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34701-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM27

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Alternative Name: TRIM27

Background:

Background Information: TRIM27 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Gene Name: Tripartite motif containing 27

Alternative Symbols: RFP, RNF76

Protein Name: Zinc finger protein RFP

Molecular Weight: 41kDa

Gene ID: 5987

NCBI Accession: NP_112212

UniProt: P14373