HSBP1 antibody (N-Term)

Catalog No. ABIN2776380

The Rabbit Polyclonal anti-HSBP1 antibody has been validated for WB. It is suitable to detect HSBP1 in samples from Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit, Zebrafish (Danio rerio) and Guinea Pig. There is 1 publication available.

Quick Overview for HSBP1 antibody (N-Term) (ABIN2776380)

Target: HSBP1 (Heat Shock Factor Binding Protein 1 (HSBP1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSBP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSBP1 Antibody

Binding Specificity: N-Term

Sequence: AETDPKTVQD LTSVVQTLLQ QMQDKFQTMS DQIIGRIDDM SSRIDDLEKN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HSBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 76 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSBP1 antibody (ABIN2776380)

Tai, McFall, Huang, Demeler, Fox, Brubaker, Radhakrishnan, Morimoto: "Structure-function analysis of the heat shock factor-binding protein reveals a protein composed solely of a highly conserved and dynamic coiled-coil trimerization domain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 735-45, (2002) (PubMed).

Target Details for HSBP1

Target: HSBP1 (Heat Shock Factor Binding Protein 1 (HSBP1))

Alternative Name: HSBP1

Background: The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.
Alias Symbols: DKFZp686D1664, DKFZp686O24200, NPC-A-13
Protein Interaction Partner: NOTCH2NL, KRTAP10-3, KRTAP10-7, LNX1, KIAA1217, CCHCR1, CCDC53, SDCBP, KIFC3, HSBP1, UBC, VCP, RRBP1, RB1CC1, HSF1, HSPA1A, HSPA4,
Protein Size: 76

Molecular Weight: 8 kDa

Gene ID: 3281

NCBI Accession: NM_001537, NP_001528

UniProt: O75506

Pathways: SARS-CoV-2 Protein Interactome