FUBP1 antibody (Middle Region)

Catalog No. ABIN2776409

This Rabbit Polyclonal antibody specifically detects FUBP1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Rabbit and Guinea Pig. It has been mentioned in 2+ publications

Quick Overview for FUBP1 antibody (Middle Region) (ABIN2776409)

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FUBP1 Antibody

Binding Specificity: Middle Region

Purpose: FUBP1 Antibody - middle region

Sequence: YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against FUBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FUBP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35704-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FUBP1 antibody (ABIN2776409)

Milosevic, Bulau, Mortz, Eickelberg: "Subcellular fractionation of TGF-beta1-stimulated lung epithelial cells: a novel proteomic approach for identifying signaling intermediates." in: Proteomics, Vol. 9, Issue 5, pp. 1230-40, (2009) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for FUBP1

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: FUBP1

Background:

Background Information: FUBP1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Far upstream element (FUSE) binding protein 1

Alternative Symbols: FBP, FUBP, hDH V

Protein Name: Far upstream element-binding protein 1

Molecular Weight: 67kDa

Gene ID: 8880

NCBI Accession: NP_003893

UniProt: Q96AE4