NMI antibody (Middle Region)

Catalog No. ABIN2776425

This Rabbit Polyclonal antibody specifically detects NMI in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig and Horse.

Quick Overview for NMI antibody (Middle Region) (ABIN2776425)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NMI Antibody

Binding Specificity: Middle Region

Purpose: NMI Antibody - middle region

Sequence: SFSKSRNGGG EVDRVDYDRQ SGSAVITFVE IGVADKILKK KEYPLYINQT

Predicted Reactivity: Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against NMI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NMI

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35737-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: NMI

Background:

Background Information: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has high expression in myeloid leukemia cell lines. NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: N-myc (and STAT) interactor

Alternative Symbols: -

Protein Name: N-myc-interactor

Molecular Weight: 35kDa

Gene ID: 9111

NCBI Accession: NP_004679

UniProt: Q13287