TIA1 antibody (N-Term)

Catalog No. ABIN2776555

The Rabbit Polyclonal anti-TIA1 antibody has been validated for WB. It is suitable to detect TIA1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse and Dog.

Quick Overview for TIA1 antibody (N-Term) (ABIN2776555)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TIA1 Antibody

Binding Specificity: N-Term

Sequence: MGKEVKVNWA TTPSSQKKDT SSSTVVSTQR SQDHFHVFVG DLSPEITTED

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TIA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TIA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 386 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA1

Background: TIA1 is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15- kDa protein that is thought to be derived from the carboxyl terminus of the 40- kDa product by proteolytic processing. The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15- kDa protein that is thought to be derived from the carboxyl terminus of the 40- kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.
Alias Symbols: TIA-1
Protein Interaction Partner: NEDD8, BMI1, SOX2, VCAM1, ITGA4, FN1, FMNL1, WDR6, SRSF3, CAND1, DCUN1D1, COPS5, CUL1, CUL2, CUL3, CUL4A, CUL5, Rc3h1, UBC, RICTOR, FASTK,
Protein Size: 386

Molecular Weight: 43 kDa

Gene ID: 7072

NCBI Accession: NM_022173, NP_071505

UniProt: P31483