FZD7 antibody (C-Term)

Catalog No. ABIN184104

Cited in 5+ publications. The Rabbit Polyclonal anti-FZD7 antibody (ABIN184104) specifically detects FZD7 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Rabbit, Pig, Guinea Pig and Saccharomyces cerevisiae samples.

Quick Overview for FZD7 antibody (C-Term) (ABIN184104)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Pig, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FZD7 Antibody

Binding Specificity: C-Term

Sequence: PDFTVFMIKY LMTMIVGITT GFWIWSGKTL QSWRRFYHRL SHSSKGETAV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%

Characteristics: This is a rabbit polyclonal antibody against FZD7. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FZD7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 574 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD7 antibody (ABIN184104)

McIver, Stanger, Santarelli, Roman, Nixon, McLaughlin: "A unique combination of male germ cell miRNAs coordinates gonocyte differentiation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35553, (2012) (PubMed).

Schmuck, Warneke, Behrens, Simon, Weichert, Röcken: "Genotypic and phenotypic characterization of side population of gastric cancer cell lines." in: The American journal of pathology, Vol. 178, Issue 4, pp. 1792-804, (2011) (PubMed).

Gökmen-Polar, Toroni, Hocevar, Badve, Zhao, Shen, Bruckheimer, Kinch, Miller: "Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer." in: Breast cancer research and treatment, Vol. 127, Issue 2, pp. 375-84, (2011) (PubMed).

Ohba, Lanigan, Roessler: "Leptin receptor JAK2/STAT3 signaling modulates expression of Frizzled receptors in articular chondrocytes." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 18, Issue 12, pp. 1620-9, (2010) (PubMed).

Ueno, Hiura, Suehiro, Hazama, Hirata, Oka, Imai, Dahiya, Hinoda: "Frizzled-7 as a potential therapeutic target in colorectal cancer." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 697-705, (2008) (PubMed).

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7

Background: FZD7 is 7-transmembrane domain protein that is receptor for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif.Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
Alias Symbols: FzE3
Protein Interaction Partner: UBC, UBQLN4, MAGI3, UBQLN1, DLG2, DLG4, DLG1,
Protein Size: 574

Molecular Weight: 63 kDa

Gene ID: 8324

NCBI Accession: NM_003507, NP_003498

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance