FZD4 antibody (Middle Region)

Catalog No. ABIN405393

Cited in 3+ publications. The Rabbit Polyclonal anti-FZD4 antibody (ABIN405393) specifically detects FZD4 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Horse and Guinea Pig samples.

Quick Overview for FZD4 antibody (Middle Region) (ABIN405393)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FZD4 Antibody

Binding Specificity: Middle Region

Purpose: FZD4 Antibody - middle region

Sequence: GITSGMWIWS AKTLHTWQKC SNRLVNSGKV KREKRGNGWV KPGKGSETVV

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against FZD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FZD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41266-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD4 antibody (ABIN405393)

Wang, Pawlak, Johnson, Belknap, Eades, Stack, Cousin, Black: "Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae." in: PLoS ONE, Vol. 8, Issue 2, pp. e56025, (2013) (PubMed).

McIver, Stanger, Santarelli, Roman, Nixon, McLaughlin: "A unique combination of male germ cell miRNAs coordinates gonocyte differentiation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35553, (2012) (PubMed).

Gonzalez, Fernandez-Martos, Gonzalez-Fernandez, Arenas, Rodriguez: "Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e50793, (2012) (PubMed).

Target Details for FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Alternative Name: FZD4

Background:

Background Information: FZD4 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence.This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Frizzled family receptor 4

Alternative Symbols: Fz4, EVR1, FEVR, Fz-4, FzE4, GPCR, hFz4, CD344, FZD4S

Protein Name: Frizzled-4

Molecular Weight: 60 kDa

Gene ID: 8322

NCBI Accession: NP_036325

UniProt: Q9ULV1

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound