CDC25B antibody (N-Term)

Catalog No. ABIN2776752

The Rabbit Polyclonal anti-CDC25B antibody is suitable to detect CDC25B in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow and Rabbit. It has been validated for WB and IHC.

Quick Overview for CDC25B antibody (N-Term) (ABIN2776752)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CDC25B Antibody

Binding Specificity: N-Term

Purpose: CDC25B Antibody - N-terminal region

Sequence: TQTMHDLAGL GSETPKSQVG TLLFRSRSRL THLSLSRRAS ESSLSSESSE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CDC25B. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDC25B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8096669

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background:

Background Information: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Gene Name: Cell division cycle 25 homolog B (S. pombe)

Protein Name: M-phase inducer phosphatase 2

Molecular Weight: 65kDa

Gene ID: 994

NCBI Accession: NP_068659

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy