MSH2 antibody (N-Term)

Catalog No. ABIN2776761

Cited in 1 publication. The Rabbit Polyclonal anti-MSH2 antibody (ABIN2776761) specifically detects MSH2 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse, Rabbit and Cow samples.

Quick Overview for MSH2 antibody (N-Term) (ABIN2776761)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MSH2 Antibody

Binding Specificity: N-Term

Purpose: MSH2 Antibody - N-terminal region

Sequence: GNKASKENDW YLAYKASPGN LSQFEDILFG NNDMSASIGV VGVKMSAVDG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MSH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MSH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41351-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MSH2 antibody (ABIN2776761)

Wedrén, Lovmar, Humphreys, Magnusson, Melhus, Syvänen, Kindmark, Landegren, Fermér, Stiger, Persson, Baron, Weiderpass: "Estrogen receptor alpha gene polymorphism and endometrial cancer risk--a case-control study." in: BMC cancer, Vol. 8, pp. 322, (2009) (PubMed).

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background:

Background Information: MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: MutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Alternative Symbols: FCC1, COCA1, HNPCC, LCFS2, hMSH2, HNPCC1, MMRCS2

Protein Name: DNA mismatch repair protein Msh2

Molecular Weight: 105kDa

Gene ID: 4436

NCBI Accession: NP_000242

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response