MUC1 antibody (Middle Region)

Catalog No. ABIN2776818

This anti-MUC1 antibody is a Rabbit Polyclonal antibody detecting MUC1 in IHC and WB. Suitable for Human, Mouse, Rat, Cow, Dog, Pig, Rabbit and Goat.

Quick Overview for MUC1 antibody (Middle Region) (ABIN2776818)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-MUC1 Antibody

Binding Specificity: Middle Region

Sequence: ASSTPGGEKE TSATQRSSVP SSTEKNAFNS SLEDPSTDYY QELQRDISEM

Predicted Reactivity: Cow: 79%, Dog: 79%, Goat: 79%, Human: 100%, Mouse: 86%, Pig: 83%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MUC1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MUC1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 159 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background: MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
Alias Symbols: CD227, EMA, H23AG, MAM6, PEM, PEMT, PUM, KL-6, MUC-1, MUC-1/X, MUC1/ZD, MUC-1/SEC
Protein Interaction Partner: UBC, PPARG, IPO5, NUP62, EGFR, ABL1, SRC, JUN, TP53, TBP, IKBKG, TNFRSF1A, IKBKB, GRB2, ESR1, GALNT15, GALNT12, GALNT10, OSGEP, GSK3B, GALNT2, GALNT1, CTNND1, SIGLEC1, ERBB4, ERBB3, SOS1, PRKCD, ZAP70, APC, JUP, LYN, ERBB2, LCK, CTNNB1, GALNT4, BAX, CDKN1
Protein Size: 159

Molecular Weight: 14 kDa

Gene ID: 4582

NCBI Accession: NM_001044392, NP_001037857

UniProt: Q7Z550

Pathways: Negative Regulation of intrinsic apoptotic Signaling