CKMT2 antibody (N-Term)

Catalog No. ABIN2776839

This Rabbit Polyclonal antibody specifically detects CKMT2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Horse, Zebrafish (Danio rerio) and Guinea Pig. It has been mentioned in 2+ publications

Quick Overview for CKMT2 antibody (N-Term) (ABIN2776839)

Target: CKMT2 (Creatine Kinase, Mitochondrial 2 (Sarcomeric) (CKMT2))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CKMT2 Antibody

Binding Specificity: N-Term

Purpose: CKMT2 Antibody - N-terminal region

Sequence: GTSVLTTGYL LNRQKVCAEV REQPRLFPPS ADYPDLRKHN NCMAECLTPA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CKMT2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CKMT2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098008

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CKMT2 antibody (ABIN2776839)

Choudhuri, Maitra, Evans: "Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 24, pp. 9818-23, (2013) (PubMed).

Guerrero, Wuyam, Mezin, Vivodtzev, Vendelin, Borel, Hacini, Chavanon, Imbeaud, Saks, Pison: "Functional coupling of adenine nucleotide translocase and mitochondrial creatine kinase is enhanced after exercise training in lung transplant skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 289, Issue 4, pp. R1144-54, (2005) (PubMed).

Target Details for CKMT2

Target: CKMT2 (Creatine Kinase, Mitochondrial 2 (Sarcomeric) (CKMT2))

Alternative Name: CKMT2

Background:

Background Information: Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80 % homology with the coding exons of ubiquitous mitochondrial creatine kinase.Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80 % homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis.

Gene Name: Creatine kinase, mitochondrial 2 (sarcomeric)

Alternative Symbols: SMTCK

Protein Name: Creatine kinase S-type, mitochondrial

Molecular Weight: 46kDa

Gene ID: 1160

NCBI Accession: NP_001816

UniProt: P17540