GSTM1 antibody (N-Term)

Catalog No. ABIN2776984

This Rabbit Polyclonal antibody specifically detects GSTM1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Goat, Sheep and has been mentioned in 2+ publications.

Quick Overview for GSTM1 antibody (N-Term) (ABIN2776984)

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GSTM1 Antibody

Binding Specificity: N-Term

Sequence: KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGAHKI TQSNAILCYI

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against GSTM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GSTM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 218 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GSTM1 antibody (ABIN2776984)

Zachut, Kra, Livshitz, Portnick, Yakoby, Friedlander, Levin: "Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows." in: Journal of proteomics, Vol. 158, pp. 52-61, (2017) (PubMed).

Bates, Li, Liang, Sarojini, An, Masternak, Bartke, Wang: "MicroRNA regulation in Ames dwarf mouse liver may contribute to delayed aging." in: Aging cell, Vol. 9, Issue 1, pp. 1-18, (2010) (PubMed).

Target Details for GSTM1

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Alternative Name: GSTM1

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. GSTM1 a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione.Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.
Alias Symbols: GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MGC26563, MU, MU-1
Protein Interaction Partner: GSTM3, UBC, IQCB1, MAP3K5, GSTM2, GSTM1,
Protein Size: 218

Molecular Weight: 26 kDa

Gene ID: 2944

NCBI Accession: NM_000561, NP_000552

UniProt: P46439

Pathways: Negative Regulation of Transporter Activity