RUNX1 antibody (Middle Region)

Catalog No. ABIN2777187

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.It has been mentioned in 1 publication

Quick Overview for RUNX1 antibody (Middle Region) (ABIN2777187)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RUNX1 Antibody

Binding Specificity: Middle Region

Purpose: RUNX1 Antibody - middle region

Sequence: ERSPPRILPP CTNASTGSAL LNPSLPNQSD VVEAEGSHSN SPTNMAPSAR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against RUNX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30954-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RUNX1 antibody (ABIN2777187)

Takeshita, Ichikawa, Nitta, Goyama, Asai, Ogawa, Chiba, Kurokawa: "AML1-Evi-1 specifically transforms hematopoietic stem cells through fusion of the entire Evi-1 sequence to AML1." in: Leukemia, Vol. 22, Issue 6, pp. 1241-9, (2008) (PubMed).

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background:

Background Information: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. RUNX1 is the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal trans

Gene Name: Runt-related transcription factor 1

Alternative Symbols: AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, CBF2alpha, AML1-EVI-1, PEBP2alpha

Protein Name: Runt-related transcription factor 1

Molecular Weight: 49kDa

Gene ID: 861

NCBI Accession: NP_001001890

UniProt: Q01196