IRF9 antibody (N-Term)

Catalog No. ABIN2777226

Cited in 2+ publications. The Rabbit Polyclonal anti-IRF9 antibody (ABIN2777226) specifically detects IRF9 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for IRF9 antibody (N-Term) (ABIN2777226)

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IRF9 Antibody

Binding Specificity: N-Term

Purpose: ISGF3G Antibody - N-terminal region

Sequence: PWKHAGKQDF REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ISGF3G. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ISGF3G

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31200-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IRF9 antibody (ABIN2777226)

Bidwell, Slaney, Withana, Forster, Cao, Loi, Andrews, Mikeska, Mangan, Samarajiwa, de Weerd, Gould, Argani, Möller, Smyth, Anderson, Hertzog, Parker: "Silencing of Irf7 pathways in breast cancer cells promotes bone metastasis through immune escape." in: Nature medicine, Vol. 18, Issue 8, pp. 1224-31, (2013) (PubMed).

Schumacher, Lau, Johnson: "Structural basis of core promoter recognition in a primitive eukaryote." in: Cell, Vol. 115, Issue 4, pp. 413-24, (2003) (PubMed).

Target Details for IRF9

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Alternative Name: ISGF3G

Background:

Background Information: ISGF3G functions to recruit RNA polymerase II to the promoter of interferon-stimulated genes and requires histone deacetylases. Defects in ISGF3 can cause resistance to IFN-.(2a) treatment.

Gene Name: Interferon regulatory factor 9

Alternative Symbols: p48, IRF-9, ISGF3, ISGF3G

Protein Name: Interferon regulatory factor 9

Molecular Weight: 44 kDa

Gene ID: 10379

NCBI Accession: NP_006075

UniProt: Q00978

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Hepatitis C